FIG 1.

Single-cell transcriptomic clustering of CD4⁺ and CD8⁺ T cells from patients with active TB and healthy donor individuals. (A) Schematic workflow design for scRNA-seq. CD4⁺ and CD8⁺ T cells were purified from PBMCs collected from three patients with active TB and three healthy donors. A 10× genomic scRNA-seq platform was used to perform transcriptomic profiling. (B and C) CD8⁺ (B) and CD4⁺ (C) T-cell subset population features. The t-SNE projection was used to visualize the nine CD8⁺ T cell subsets. Each dot represents a single cell colored according to the cell clustering information. (D and E) Heatmaps showing the top marker genes across each CD8⁺ (D) and CD4⁺ (E) T-cell cluster. (F and G) Heatmaps showing the pairwise Pearson correlation coefficients of T-cell subsets for CD8⁺ T cells (F) and CD4⁺ T cells (G).