TABLE 2.
Virulence factors of E. coli isolates 1 and 2 compared to a classical neonatal meningitis E. coli strain
| Function | Virulence factora | Related gene(s) | Isolate 1 | Isolate 2 | O18:K1 NMECST95 (IHE3034)b |
|---|---|---|---|---|---|
| Adherence | E. coli common pilus | ecpR | − | + | + |
| ecpA | + | + | + | ||
| ecpB | + | + | + | ||
| ecpC | + | + | + | ||
| ecpD | + | + | + | ||
| cpE | + | + | + | ||
| EaeH | eaeH | + | + | + | |
| F1C fimbriae | focI | + | − | − | |
| focC | + | − | − | ||
| focD | + | − | − | ||
| focF | − | − | − | ||
| focG | + | − | − | ||
| focH | − | − | − | ||
| Hemorrhagic E. coli pilus | hcpA | + | + | + | |
| hcpB | + | + | + | ||
| hcpC | + | + | + | ||
| P fimbriae | papX | − | − | − | |
| papG | + | − | − | ||
| papF | − | − | − | ||
| papE | − | − | − | ||
| papK | − | − | − | ||
| papJ | − | − | − | ||
| papD | + | − | − | ||
| papC | + | − | − | ||
| papH | + | − | − | ||
| papA | − | − | − | ||
| papB | − | − | − | ||
| papI | + | − | − | ||
| S fimbriae | sfaC | + | − | + | |
| sfaB | − | − | + | ||
| sfaA | − | − | + | ||
| sfaD | − | − | + | ||
| sfaE | − | − | + | ||
| sfaF | − | − | + | ||
| sfaG | − | − | + | ||
| sfaS | − | − | + | ||
| sfaH | − | − | + | ||
| Type I fimbriae | fimB | − | − | + | |
| fimE | + | + | + | ||
| fimA | + | + | + | ||
| fimI | + | + | + | ||
| fimC | + | + | + | ||
| fimD | + | + | + | ||
| fimf | + | + | + | ||
| fimG | + | + | + | ||
| fimH | + | + | + | ||
| Autotransporter | Contact-dependent inhibition system | cdiA | + | − | − |
| cdiB | + | − | − | ||
| EhaB | ehaB | + | + | + | |
| EspC | espC | + | − | − | |
| Temperature-sensitive hemagglutinin | tsH | + | − | − | |
| UpaG adhesin | upaG/ehaG | + | − | + | |
| Cah | cah | − | + | + | |
| Immunoglobulin repeat protein | air/eaeX | − | + | − | |
| Vacuolating autotransporter gene | vat | − | + | + | |
| Invasion | Invasion of brain endothelial cells | ibeA | + | − | + |
| ibeB | + | + | + | ||
| ibeC | + | + | + | ||
| Tia/Hek | tia | + | − | − | |
| Iron uptake | Heme uptake | chuS | + | + | + |
| chuA | + | + | + | ||
| chuT | + | + | + | ||
| chuW | + | + | + | ||
| chuX | + | + | + | ||
| chuY | + | + | + | ||
| chuU | + | + | + | ||
| Aerobactin siderophore | iutA | − | + | − | |
| iucD | − | + | − | ||
| iucC | − | + | − | ||
| iucB | − | + | − | ||
| iucA | − | + | − | ||
| Iron-regulated element | ireA | + | − | − | |
| Iron/manganese transport | sitA | − | − | + | |
| sitB | − | − | + | ||
| sitC | − | − | + | ||
| sitD | − | + | + | ||
| Iron/manganese transport | iroN | + | + | + | |
| iroE | + | + | + | ||
| iroD | + | + | + | ||
| iroC | + | + | + | ||
| iroB | + | + | + | ||
| Yersiniabactin siderophore | ybtS | + | + | + | |
| ybtX | + | + | + | ||
| ybtQ | + | + | + | ||
| ybtP | + | + | + | ||
| ybtA | + | + | + | ||
| irp2 | + | + | + | ||
| irp+ | + | + | + | ||
| ybtU | + | + | + | ||
| ybtE | + | + | + | ||
| fyuA | + | + | + | ||
| Toxin | Alpha hemolysin | hlyC | − | − | − |
| hlyA | + | − | − | ||
| hlyB | + | − | − | ||
| hlyD | + | − | − | ||
| Colicin-like Usp | usp | + | − | − | |
| Cytotoxic necrotizing factor 1 | cnf1 | + | − | − | |
| Hemolysin/cytolysin A | hlyE/clyA | Partial | + | − | |
| Cytolethal distending toxin | cdtA | + | − | + | |
| cdtB | + | − | + | ||
| cdtC | − | − | + | ||
| Non-LEE-encoded T3SSc | Only genes present in our sequences | espL1 | − | + | − |
| espL4 | − | + | − | ||
| espR1 | − | + | − | ||
| espX1 | − | + | − | ||
| espX4 | − | + | − | ||
| espX5 | − | + | − | ||
| espY1 | − | + | − | ||
| espY2 | − | + | − | ||
| espY3 | − | + | − | ||
| Secretion system | ACE T6SS (only genes present in our sequences) | aec32 | − | + | + |
| aec31 | − | + | + | ||
| aec30 | − | + | + | ||
| aec29 | − | + | + | ||
| aec28 | − | + | + | ||
| aec27/clpV | − | + | + | ||
| aec26 | − | + | + | ||
| aec25 | − | + | + | ||
| aec24 | − | + | + | ||
| aec22 | − | + | + | ||
| aec19 | − | + | + | ||
| aec18 | − | + | + | ||
| aec17 | − | + | + | ||
| aec16 | − | + | + | ||
| aec15 | − | + | + | ||
| aec14 | − | − | + | ||
| aec11 | − | − | + | ||
| aec8 | − | − | + | ||
| aec7 | − | − | + |
a
E. coli common pilus (ECP), hemorrhagic E. coli pilus (HCP), contact-dependent inhibition (CDI) system, invasion of brain endothelial cells (Ibes).
b
NMEC, neonatal meningitis E. coli. Light gray shading indicates the absence of a gene; dark gray shading indicates the gene’s presence.
c
Non-LEE-encoded T3SS (type 3 secretion system): only genes present in our sequences.