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. 2023 Feb 6;11(2):e01583-22. doi: 10.1128/spectrum.01583-22

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Copyright © 2023 Ramesh et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 8 — Flow cytometric analysis of reactive oxygen species (ROS) generation upon treatment. (A) FC histograms of C. tropicalis ATCC 750 cells stained with DCFH-DA (2′,7′-dichlorodihydrofluorescein diacetate) analyzed using an FITC (fluorescein isothiocyanate) filter. Panels showing percentage of DCFH-DA-positive cells (FL1), indicating the ROS generated by (i) unstained cells, (ii) untreated cells, and cells treated with (iii) 2 mg/L AMB, (iv) 4 mg/L AMB, (v) 8 mg/L AF₄, (vi) 16 mg/L AF₄, or (vii) 10 mM H₂O₂. (B) FC histograms showing traces of C. auris IL-3331 cells stained with DCFH-DA analyzed using an FITC filter. Percentage of DCFH-DA positive cells (FL1) indicating the ROS generated by (i) unstained cells, (ii) untreated cells, and cells treated with (iii) 4 mg/L AMB, (iv) 8 mg/L AMB, (v) 8 mg/L AF₄, (vi) 16 mg/L AF₄, or (vii) 10 mM H₂O₂ (C) Percentage of ROS-positive cells in C. tropicalis and C. auris across treatments. Results represent mean ± SD and P values (P = 0.0014 for C. tropicalis and P < 0.0001 for C. auris) denote statistically significant differences compared to control assessed by one-way ANOVA. A total of 20,000 events were analyzed.