TABLE 2.
Possible interactions for each microbial pair, based on the results from the three mechanistic assays (CFS effect on Bd growth from bacteria grown together, separate-then-combined CFS, and bacterial isolates grown together and separately)a
| Microbial pair | Summary of interactions | Outcome |
|---|---|---|
| Pseudomonas LC2F and Microbacterium CW37E | No enhanced growth rate, facilitative effect, or metabolite effect when grown together | Pseudomonas LC2F displayed only slightly stronger Bd inhibition than did Microbacterium CW37E, and there was no measured interaction between the two |
| Pseudomonas LC2F and Curvibacter CW54D | No enhanced growth rate, facilitative effect, or metabolite effect when grown together | Curvibacter CW54D did not affect the ability of Pseudomonas LC2F to inhibit Bd |
| Pseudomonas LC2F and Collimonas TK10 | No enhanced growth rate, facilitative effect, or metabolite effect when grown together | Pseudomonas LC2F displayed stronger Bd inhibition than did Collimonas TK10, and there was no measured interaction between the two |
| Pseudomonas LC2F and J. lividum (str. BTP) | No enhanced growth rate, facilitative effect, or metabolite effect when grown together | Pseudomonas LC2F displayed stronger Bd inhibition than did J. lividum (str. BTP), and there was no measured interaction between the two |
| Chryseobacterium CW20D and Pseudomonas LC2F | Enhanced each other’s growth rate but did not have a metabolite or facilitative effect on Bd | These microbes may be interacting with each other, but this interaction does not increase Bd inhibition in any way |
| Chryseobacterium CW20D and Microbacterium CW37E | No enhanced growth rate, facilitative effect, or metabolite effect when grown together | These microbes did not change each other’s' ability to inhibit Bd when grown together |
| Chryseobacterium CW20D and Curvibacter CW54D | Enhanced each other’s' growth rate and increased Bd growth when their metabolites were together; the metabolite effect of Chryseobacterium CW20D on Bd disappeared when grown with Burkholderiales | Curvibacter CW54D may be competing with Chryseobacterium CW20D for space or resources; Chryseobacterium CW20D no longer puts resources into producing anti-Bd metabolite(s), which enhances Bd growth by some unknown mechanism |
| Chryseobacterium CW20D and Collimonas TK10 | Enhanced each other’s growth rate but did not have a metabolite or facilitative effect on Bd | These microbes may be interacting with each other, but this interaction does not increase Bd inhibition in any way |
| Chryseobacterium CW20D and J. lividum (str. BTP) | No enhanced growth rate, facilitative effect, or metabolite effect when grown together | Chryseobacterium CW20D displayed stronger Bd inhibition than did J. lividum (str. BTP), and there was no measured interaction between the two |
| Curvibacter CW54D and Microbacterium CW37E | No enhanced growth rate, facilitative effect, or metabolite effect when grown together | Curvibacter CW54D did not affect the ability of Microbacterium CW37E to inhibit Bd |
| Curvibacter CW54D and Collimonas TK10 | Collimonas TK10 likely outgrew Curvibacter CW54D when grown together; a facilitative interaction was observed | These two microbes may induce a metabolite response from each other through competition, which subsequently inhibits Bd |
| Curvibacter CW54D and J. lividum (str. BTP) | J. lividum (str. BTP) grew less rapidly when grown with Curvibacter CW54D, and they exhibited an additive metabolite response | These two microbes may induce a metabolite response from each other through competition, which subsequently inhibits Bd |
| Collimonas TK10 and Microbacterium CW37E | No enhanced growth rate, facilitative effect, or metabolite effect when grown together | Microbacterium CW37E displayed stronger Bd inhibition than did Collimonas TK10, and there was no measured interaction between the two |
| Collimonas TK10 and J. lividum (str. BTP) | No enhanced growth rate, facilitative effect, or metabolite effect when grown together | J. lividum (str. BTP) displayed stronger Bd inhibition than did Collimonas TK10, and there was no measured interaction between the two |
| J. lividum (str. BTP) and Microbacterium CW37E | J. lividum (str. BTP) grew more rapidly alone than with Microbacterium CW37E, but there was no enhanced metabolic effect; Microbacterium CW37E inhibited Bd better than did J. lividum (str. BTP) | Microbacterium CW37E may be competing with J. lividum (str. BTP) for space or resources and inhibits Bd better |
a
The summary of interactions is based primarily on Fig. S1, S2, and S3.