TABLE 2.
Plasmids used in this study
| Plasmid | Features | Source |
|---|---|---|
| pSK bluescript | Bluescript plasmid with AmpR | Agilent |
| pSK-pECE1500-GFP-SAT1 | pSK, pECE1500 fused to GFP, SAT1 as selection marker, and homology regions for integration into CaNEUT5L locus | This work |
| pSK-pECE11000-GFP-SAT1 | pSK, pECE11000 fused to GFP, SAT1 as selection marker, and homology regions for integration into CaNEUT5L locus | This work |
| pSK-pECE11500-GFP-SAT1 | pSK, pECE11500 fused to GFP, SAT1 as selection marker, and homology regions for integration into CaNEUT5L locus | This work |
| pSK-pECE12000-GFP-SAT1 | pSK, pECE12000 fused to GFP, SAT1 as selection marker, and homology regions for integration into CaNEUT5L locus | This work |
| pSK-pECE12500-GFP-SAT1 | pSK, pECE12500 fused to GFP, SAT1 as selection marker, and homology regions for integration into CaNEUT5L locus | This work |
| pSK-pECE13000-GFP-SAT1 | pSK, pECE13000 fused to GFP, SAT1 as selection marker, and homology regions for integration into CaNEUT5L locus | This work |
| pSK-pECE1T1Mut-GFP-SAT1 | pSK, pECE11500 with mutated TATA box 1 (−109), fused to GFP, SAT1 as selection marker, and homology regions for integration into CaNEUT5L locus | This work |
| pSK-pECE1T2Mut-GFP-SAT1 | pSK, pECE11500 with mutated TATA box 2 (−284), fused to GFP, SAT1 as selection marker, and homology regions for integration into CaNEUT5L locus | This work |