TABLE 3.
Oligonucleotide primers used in this study
| Name | Sequence in 5′ to 3′ directiona |
|---|---|
| 5′GFP-XhoI | AGCTctcgagATGAGTAAGGGAGAAGAACTTTTCACTGGA |
| 3'GFP-EcoRV | AGCTgatatcTTATTTGTATAGTTCATCCATGCCATGTGT |
| 5′CaACT1term-EcoRI | AGCTgaattcGAGTGAAATTCTGGAAATCTGGAAATCT |
| 3'CaACT1term-SpeI | AGCTactagtTAGATTATGGTCGACATTTTATGATGGAAT |
| 5′NEUfwd-ApaI | ACTGgggcccGTAATTGTAGTAAGAATGACAAGTATCAG |
| 5'NEUrev-ANP-XhoI | ACTGctcgagttaattaaggcgccggcgcgccGGAAGGACGATGAAGGAGAGAAAG |
| 3′NEUfwd-SacII | ACTGccgcggTAAACAAGTGGTATTCAAGCACAATTC |
| 3'NEUT5L-SacIPacI | GCTGgagctcttaattaaTAACCCACTGAATTCTACATCGAAC |
| 3'ECE1prom-XhoI | TCATctcgagTTTAACGAATGGAAAATAGTTGGTAGTAAAATAAAGG |
| 5′ECE1prom500-AscI | TTCCggcgcgccGTCATTTGTAGGATTTTCAGCAGAACA |
| 5′ECE1prom1000-AscI | TTCCggcgcgccAGCGAAACATTTTTTTTTTTCAACGGCTC |
| 5′ECE1prom1500-AscI | TTCCggcgcgccACCCTAGTAATTATATGAAACATGCCCA |
| 5′ECE1prom2000-AscI | TTCCggcgcgccAACATTAACGACGCAAAATACAAACTTGGT |
| 5′ECE1prom2500-AscI | TTCCggcgcgccGTAAGCATTTTGGAGTAATACCATAGTTG |
| 5′ECE1prom3000-AscI | TTCCggcgcgccGCAAATAGAATTGTTCTATTGCTTAGCTTTAG |
| R1-CaACT1 | TCAGACCAGCTGATTTAGGTTTG |
| R2-CaACT1 | GTGAACAATGGATGGACCAG |
| R1-CaECE1 | ATCGAAAATGCCAAGAGAG |
| R2-CaECE1 | AGCATTTTCAATACCGACAG |
| R1-CaGFP | CTGAAGTCAAGTTTGAAGGTGATAC |
| R2-CaGFP | GCAGATTGTGTGGACAAGTAATG |
| GFP veri rev | TGATCTGGGTATCTCGCAAAGCAT |
a
Lowercase: restriction sites.