TABLE 1.
Information on the CoV proteins included in the Luminex multiplex serology assaya
| Recombinant protein | Region (aa) | Expression system | Full name of protein | Reporting units |
|---|---|---|---|---|
| SARS-CoV-2-S | 14–1213 | Expi293F cells | SARS-CoV-2 S protein | BAU/mL |
| SARS-CoV-2-N | 1–419 | E. coli BL21(DE3) | SARS-CoV-2 N protein | BAU/mL |
| SARS-CoV-2-RBD (R) | 319–529 | Expi293F cells | SARS-CoV-2 RBD | BAU/mL |
| SARS-CoV-2-RBD (N501Y) mutant (R) | 318–529 | Expi293F cells | SARS-CoV-2 RBD (B.1.1.7) | BAU/mL |
| SARS-CoV-2-RBD (E484K) mutant (R) | 318–529 | Expi293F cells | SARS-CoV-2 RBD (E484K) | BAU/mL |
| SARS-CoV-2-RBD (M) | 319–541 | Expi293F cells | SARS-CoV-2 RBD | BAU/mL |
| SARS-CoV-2-RBD (N501Y) mutant (M) | 319–541 | Expi293F cells | SARS-CoV-2 RBD (B.1.1.7) | BAU/mL |
| SARS-CoV-2-RBD (K417N/E484K/N501Y) triple mutant (M) | 319–541 | Expi293F cells | SARS-CoV-2 RBD (B.1.351) | BAU/mL |
| SARS-CoV-2-RBD (E484K) mutant (M) | 319–541 | Expi293F cells | SARS-CoV-2 RBD (E484K) | BAU/mL |
| SARS-CoV-1-S | 14–1190 | Expi293F cells | SARS-CoV-1 S protein | AU/mL |
| MERS-S | 18–1291 | Expi293F cells | MERS S protein | IU/mL |
| HCoV-OC43 | 15–1278 | Expi293F cells | Human coronavirus OC43 S protein | AU/mL |
| HCoV-229E | 16–1110 | Expi293F cells | Human coronavirus 229E S protein | AU/mL |
| HCoV-HKU1 | 14–1276 | Expi293F cells | Human coronavirus HKU1 S protein | AU/mL |
| HCoV-NL63 | 15–1291 | Expi293F cells | Human coronavirus NL63 S protein | AU/mL |
a
Mount Sinai (M) and Ragon (R) proteins differ in their C-terminal tags and construct signal peptides.