TABLE 4.
Intra/interday and interanalyst precisiona
| Assayb | No. of measurements | % RSD |
ICC | |||
|---|---|---|---|---|---|---|
| Mean intraday | Mean interday | Mean analyst | Total | |||
| CoV-2 S | 480 | 11.6 | 2.8 | 5.5 | 13.1 | 99.3 |
| CoV-2 N | 240 | 11.6 | 5.1 | 4.1 | 13.3 | 98.4 |
| CoV-1 S | 480 | 13.8 | 1.8 | 2.7 | 14.1 | 99.1 |
| MERS CoV S | 478 | 15.6 | 4.8 | 2.7 | 16.6 | 99.1 |
| OC43 CoV S | 480 | 11.3 | 3.4 | 5.2 | 12.9 | 99.1 |
| 229E CoV S | 480 | 10.8 | 3.4 | 3.9 | 12.0 | 99.4 |
| HKU1 CoV S | 480 | 11.7 | 1.5 | 5.2 | 12.9 | 98.9 |
| NL63 CoV S | 480 | 11.3 | 3.3 | 3.9 | 12.4 | 99.1 |
| RBD WT (R) | 480 | 11.7 | 6.4 | 3.4 | 13.8 | 99.3 |
| RBD mutant N501Y (R) | 480 | 11.7 | 5.1 | 4.9 | 13.7 | 99.3 |
| RBD mutant E484K (R) | 480 | 11.4 | 3.8 | 3.6 | 12.6 | 99.6 |
| RBD WT (M) | 480 | 12.1 | 3.4 | 3.6 | 13.1 | 99.3 |
| RBD mutant N501Y (M) | 480 | 11.8 | 4.2 | 3.6 | 13.0 | 99.3 |
| RBD triple mutant (M) | 480 | 11.6 | 4.1 | 2.8 | 12.7 | 99.6 |
| RBD mutant E484K (M) | 480 | 11.5 | 3.0 | 3.2 | 12.3 | 99.6 |
a
A percent error ≤25% threshold was used to define at which concentrations the assay may be deemed acceptable. Data include replicates and % relative standard deviation (RSD) or CV and intraclass correlation (ICC), which is a measure of true variance to total observed variance.
b
Protein sequences were associated with Mount Sinai (M) and Ragon (R).