TABLE 2.
Primers used in this studya
| Primer name | Element, cut site | Sequence |
|---|---|---|
| JCO70 | Screening GFP insertion | ATGAGCAAAGGCGAAGAAC |
| JCO71 | Screening GFP insertion | CAGTTCATCCATGCCATGT |
| EPO61 | oriT, PciI | AAAAACATGTGCTTGGTTTCATCAGCC |
| EPO62 | oriT, PciI | AAAAACATGTCTGCTTCGGGGTCATTATAG |
| EPO92 | Screening transconjugants | GTCTCATGAGCGGATACATAT |
| EPO93 | Screening transconjugants | CCATATGTTGCATCACCTT |
| EPO114 | pAprE, AatII | AAAAGACGTCGCATTTCGGGTATCGAATG |
| EPO110 | pAprE, XbaI | AAAATCTAGACTTATTTCAGAATAATCATCCG |
| EPO165 | pSpoIIGA, AatII | AAAAGACGTCCGATATTCTTTCTGTTTCATACG |
| EPO166 | pSpoIIGA, XbaI | AAAATCTAGAGTTTGTACCAGTATAAAACAGTC |
| qEPO61 | ftsZ (qPCR) | GACATGGTCTTTGTGACAGCC |
| qEPO62 | ftsZ (qPCR) | GTGAAAGGACGAGTCACAACTC |
| qEPO117 | gfp (qPCR) | CAATGCCGGAAGGCTATG |
| qEPO118 | gfp (qPCR) | GCGATTGACCAGTGTATCGC |
| 27F | 16S sequencing | AGAGTTTGATCMTGGCTCAG |
| 1522R | 16S sequencing | AAGGAGGTGATCCANCCRCA |
a
Cut sites are shown in bold.