FIG 4.

Production of fluorescent S. brasiliensis tagged strains with the pGAPDH promoter. (A) A representative scheme of the toolkit produced showing all the minimal modules composing the binary vectors, including the two promoters (pGAPDH and pH2A), the AvrII restriction cloning site, the translation starting codon, indicating the open reading frame, inert-linker, and sGFP or mCherry segments. (B) Fluorescence microscopic analysis of the S. brasiliensis strains, expressing sGFP or mCherry in-frame fused to the C-terminal of the H2A. The merged images show a high degree of co-localization between sGFP and mCherry expressions with the DAPI (4[prime],6-diamidino-2-phenylindole) nuclei staining during both yeast and mycelium phases. The scale bar equals 10 μm for all images.