TABLE 1.
Mosquito RNA viruses discovered in this study
| Virus no. | Classification | Virus namea | Segment no. | Length (nt) | Closest relative | RdRp aa identity (%) | RPMb |
|---|---|---|---|---|---|---|---|
| dsRNA viruses | |||||||
| 1 | Chrysoviridae related | Shuangao chryso-like virus 1 YN2018 | 4 | Shuangao chryso-like virus 1 strain mosWSB68555 | 100 | 140 | |
| 2 | Partitiviridae related | Aedes partiti-like virus 1 YN2018 | 2 | Aedes partiti-like virus 1 isolate Jane | 100 | 2,539 | |
| 3 | Partitiviridae related | Aedes partiti-like virus 2* | 1 | 1,709 | Culex tritaeniorhynchus partitivirus 17CxITNGK-Ctr | 61.17 | 1,770 |
| 4 | Partitiviridae related | Armigeres partiti-like virus 1* | 1 | 1,486 | Aedes partiti-like virus 1 isolate Jane | 83.47 | 57 |
| 5 | Partitiviridae related | Sonnbo virus YN2018 | 3 | Sonnbo virus isolate OTU64 | 99.46 | 179 | |
| 6 | Partitiviridae related | Verdadero virus YN2018 | 2 | Verdadero virus isolate Vv.PozaRica20 | 99.62 | 663 | |
| 7 | Reoviridae related | Aedes reo-like virus 1* | 2 | 4,216/3,096 | Shenzhen reo-like virus 2 | 30.00 | 922 |
| 8 | Totiviridae related | Aedes aegypti toti-like virus YN2018 | 1 | Aedes aegypti toti-like virus strain 2017-PB-AAM-5 | 98.70 | 32 | |
| 9 | Totiviridae related | Aedes aegypti totivirus YN2018 | 1 | Aedes aegypti totivirus GH115 | 97.16 | 289 | |
| 10 | Totiviridae related | Culex toti-like virus 1* | 1 | 7,194 | Fitzroy Crossing toti-like virus 2 isolate FCToLV2/pool-7 | 73.20 | 81 |
| −ssRNA viruses | |||||||
| 11 | Bunyavirales | Aedes bunya-like virus 1* | 1 | 7,731 | Salarivirus Mos8CM0 | 48.54 | 196 |
| 12 | Bunyavirales | Barstukas virus YN2018 | 3 | Barstukas virus isolate CMS002_026c_WVAL | 99.54 | 146 | |
| 13 | Bunyavirales | Culex bunyavirus 1 YN2018 | 1 | Bunyavirales sp. strain YX392 | 96.06 | 683 | |
| 14 | Bunyavirales | Culex quinquefasciatus bunyavirus 1* | 3 | 7,102/3,676/1,642 | Qingnian mosquito virus strain YC179 | 88.78 | 271 |
| 15 | Bunyavirales | Culex phasma-like virus YN2018 | 3 | Culex phasma-like virus strain mos191gb30698 | 99.91 | 627 | |
| 16 | Bunyavirales | Phasi Charoen-like phasivirus YN2018 | 3 | Phasi Charoen-like phasivirus strain Zhanjiang01 | 99.28 | 4,271 | |
| 17 | Bunyavirales | Zhee mosquito virus YN2018 | 1 | Zhee mosquito virus strain XC1-8 | 99.22 | 196 | |
| 18 | Mononegavirales | Aedes rhabdovirus 1* | 1 | 10,320 | Merida virus isolate CC_H | 48.08 | 21 |
| 19 | Mononegavirales | Aedes albopictus anphevirus YN2018 | 1 | Aedes albopictus anphevirus strain USA | 99.46 | 5,462 | |
| 20 | Mononegavirales | Aedes anphevirus YN2018 | 1 | Aedes anphevirus isolate Nakhon Nayok | 99.71 | 344 | |
| 21 | Mononegavirales | Armigeres rhabdo-like virus 2* | 1 | 10,143 | San Gabriel mononegavirus isolate San Gabriel Valley | 52.71 | 37 |
| 22 | Mononegavirales | Armigeres rhabdo-like virus 1* | 1 | 12,152 | Formosus virus isolate U30 | 72.52 | 169 |
| 23 | Mononegavirales | Culex rhabdo-like virus 2* | 1 | 11,680 | Culex rhabdo-like virus strain CRVL/Los Angeles | 73.38 | 637 |
| 24 | Mononegavirales | Culex mononega-like virus 2 YN2018 | 1 | Culex mononega-like virus 2 strain mos191X56446 | 99.76 | 26 | |
| 25 | Mononegavirales | Culex quinquefasciatus rhabdo-like virus 1 | 1 | 11,508 | Culex pseudovishnui rhabdo-like virus 17NGK-Cps-217 | 94.67 | 1,149 |
| 26 | Mononegavirales | San Gabriel mononegavirus YN2018 | 1 | San Gabriel mononegavirus isolate San Gabriel Valley | 98.67 | 93 | |
| 27 | Orthomyxoviridae related | Aedes orthomyxo-like virus 2 YN2018 | 4 | Aedes orthomyxo-like virus 2 isolate Italy Rome | 99.35 | 409 | |
| 28 | Orthomyxoviridae related | Armigeres orthomyxo-like virus 1* | 5 | 2,437/2,425/2,218/1,835/1,509 | Usinis virus isolate CMS002_026e_WVAL | 69.27 | 437 |
| 29 | Orthomyxoviridae related | Wuhan mosquito virus 6 YN2018 | 6 | Wuhan mosquito virus 6 strain YX592 | 100 | 1,659 | |
| 30 | Orthomyxoviridae related | Guadeloupe mosquito quaranja-like virus 1 YN2018 | 7 | Guadeloupe mosquito quaranja-like virus 1 isolate CMS002_017a_SAND | 99.35 | 25 | |
| 31 | Orthomyxoviridae related | Usinis virus YN2018 | 8 | Usinis virus isolate CMS002_026e_WVAL | 99.62 | 820 | |
| 32 | Orthomyxoviridae related | Wuhan mosquito virus 4 YN2018 | 5 | Wuhan mosquito virus 4 strain XC3-4 | 100 | 565 | |
| +ssRNA viruses | |||||||
| 33 | Flaviviridae related | Aedes flavivirus YN2018 | 1 | Aedes flavivirus strain Bangkok | 99.67 | 287 | |
| 34 | Flaviviridae related | Cell fusing agent virus YN2018 | 1 | Cell fusing agent virus isolate KPP | 99.19 | 107 | |
| 35 | Flaviviridae related | Culex hepacivirus 1* | 1 | 8,997 | Jogalong virus isolate P1-10 | 54.38 | 11 |
| 36 | Narnaviridae related | Zhejiang mosquito virus 3 YN2018 | 1 | Zhejiang mosquito virus 3 strain mosHB236413 | 95.23 | 485 | |
| 37 | Narnaviridae related | Hubei mosquito virus 3 YN2018 | 1 | Hubei mosquito virus 3 strain 3mos6141 | 98.2 | 806 | |
| 38 | Narnaviridae related | Mosquito narna-like virus 1* | 1 | 3,160 | Ochlerotatus-associated narna-like virus 2 isolate acc_9.11s | 36.17 | 114 |
| 39 | Negevirus related | Aedes albopictus negev-like virus YN2018 | 1 | Aedes albopictus negev-like virus isolate Aag2.wAlbB | 99.12 | 399 | |
| 40 | Negevirus related | Culex quinquefasciatus negev-like virus 1* | 1 | 10,894 | Parry's Creek negev-like virus 1 isolate PCNLV1/pool-12 | 90.05 | 1,132 |
| 41 | Solemoviridae related | Aedes sobemo-like virus 1* | 1 | 2,782 | Hubei sobemo-like virus 41 strain spider133517 | 87.6 | 11 |
| 42 | Solemoviridae related | Guadeloupe mosquito virus YN2018 | 2 | Guadeloupe mosquito virus isolate CMS002_019a_SAND | 99.55 | 3,013 | |
| 43 | Solemoviridae related | Guangzhou sobemo-like virus YN2018 | 2 | Guangzhou sobemo-like virus strain 18-GZ-adult | 99.77 | 5,457 | |
| 44 | Solemoviridae related | Hubei sobemo-like virus 41 YN2018 | 1 | Hubei sobemo-like virus 41 strain spider133517 | 99.19 | 4 | |
| 45 | Solemoviridae related | Humaita Tubiacanga virus YN2018 | 2 | Humaita-Tubiacanga virus strain Ab-AAF | 99.21 | 1,572 | |
| 46 | Solemoviridae related | Mosquito sobemo-like virus 1* | 2 | 3,055/1,626 | Guadeloupe mosquito virus isolate CMS002_019a_SAND | 83.45 | 296 |
| 47 | Tombusviridae related | Culex tombus-like virus 1* | 1 | 3,776 | Dansoman virus strain INNplus3391 | 48.81 | 385 |
| 48 | Tymoviridae related | Culex quinquefasciatus tymo-like virus 1* | 1 | 7,859 | Culex pseudovishnui tymo-like virus 17CxNGK-Cps2-1535 | 87.01 | 58 |
| 49 | Tymoviridae related | Guadeloupe Culex tymo-like virus YN2018 | 1 | Guadeloupe Culex tymo-like virus isolate CMS002_022a_SAND | 96.74 | 47 | |
| 50 | Virgaviridae related | Aedes binegev-like virus 2 YN2018 | 2 | Aedes binegev-like virus 2 isolate Shenzhen | 99.79 | 387 | |
| 51 | Virgaviridae related | Hubei virga-like virus 2 YN2018 | 1 | Hubei virga-like virus 2 isolate CMS001_019_ALCO | 98.37 | 328 | |
| 52 | Permutotetraviridae related | Sarawak virus YN2018 | 1 | Sarawak virus strain SWK-M26 | 97.23 | 6,826 |
a
Asterisks indicate novel viruses identified in this study.
b
RPM, reads per million. The largest one is shown if detected in multiple libraries.