. 2023 Feb 6;11(2):e03082-22. doi: 10.1128/spectrum.03082-22

TABLE 3.

Primer sets used during qPCR analysis of chickpea defensive genes

Gene ID	Gene name	Function	Primers	Reference
EU529707	ACT1	Housekeeping gene	F-GCCTGATGGACAGGTGATCAC	35
	ACT1	Housekeeping gene	R-GGAACAGGACCTCTGGACATCT	35
	CHI1	Hydrolysis of chitin (main component of fungal cell wall)	F-AAGGCTTTGGTGCAACCATT	Designed by using Primer3 v.0.4.0 (36)
	CHI1	Hydrolysis of chitin (main component of fungal cell wall)	R-CACCAGGAGCAACACCCAAT	Designed by using Primer3 v.0.4.0 (36)
CV793598	PR2B	β-1,3-Glucanase—hydrolysis of flavonoid and isoflavonoid compounds	F-GCCTAGAAAGGCAAATCCTTC	37
CV793598	PR2B		R-CATCTGCCGTGGGAATAAGA	37
DY475248	PAMP	Transcription of defense-related genes—resistance response via metabolism utilizing polyamines and nicotianamines	F-CCGCTGATACAGTGGAGGTT	38
DY475248	PAMP		R-GTTTCCCCAATTTCCTCACC	38
TF1070.m00005	TF1063	Host defense gene (QTL_AR1–LG4)	F-GTTATGTGGGTGGAGTTGGAA	39
TF1070.m00005	TF1063	Host defense gene (QTL_AR1–LG4)	R-CAACCATAGCTGCAACCATCT	39
TC101530	TF1082	Host defense gene (QTL_AR2–LG4)	F-AAGTCTTATCGTGGCGTTCG	38
	TF1082	Host defense gene (QTL_AR2–LG4)	R-TCATAAGCTAGTGCTGCTGCT	38
	PAL	Induced phenyl propanoid pathway	F-AAGCTCACTTGCAGAGACAT R-ACCTGATGCAGTGATTGTGC	40