TABLE 5.
Principal-component analysis of inflammatory parameters, clinical data, and the top bacterial species in sputum of 28 PI CF patientsa
| Parameter | Contribution (%) of observation in dimension |
||||||||
|---|---|---|---|---|---|---|---|---|---|
| F1 | F2 | F3 | F4 | F5 | F6 | F7 | F8 | F9 | |
| Streptococcus mitis | 9.1 | 0.9 | 1.4 | 2.6 | 0.9 | 0.5 | 0.3 | 0.5 | 0.4 |
| Streptococcus gordonii | 8.0 | 0.0 | 0.0 | 0.1 | 0.1 | 1.8 | 1.0 | 4.8 | 0.1 |
| Neisseria meningitidis | 4.6 | 2.4 | 1.7 | 0.4 | 0.0 | 0.2 | 2.6 | 0.1 | 2.6 |
| Haemophilus parainfluenzae | 5.1 | 2.3 | 1.7 | 0.2 | 0.0 | 0.1 | 5.4 | 0.2 | 3.2 |
| Rothia mucilaginosa | 3.4 | 0.5 | 0.1 | 0.2 | 0.0 | 7.3 | 5.6 | 1.3 | 0.5 |
| NE | 4.0 | 1.8 | 2.7 | 3.0 | 1.7 | 4.2 | 1.1 | 0.4 | 0.9 |
| Streptococcus oralis | 7.0 | 0.1 | 1.8 | 3.5 | 0.0 | 1.1 | 1.1 | 0.4 | 0.1 |
| Streptococcus pseudopneumoniae | 8.3 | 1.0 | 1.3 | 3.4 | 1.1 | 1.3 | 0.1 | 0.5 | 0.1 |
| Streptococcus pneumoniae | 7.7 | 0.8 | 1.3 | 4.2 | 1.1 | 1.6 | 0.0 | 0.7 | 0.0 |
| Staphylococcus epidermidis | 2.8 | 0.2 | 0.5 | 5.6 | 7.4 | 0.0 | 0.7 | 1.1 | 0.1 |
| Cutibacterium acnes | 2.9 | 1.2 | 0.0 | 0.1 | 9.2 | 0.4 | 2.3 | 1.9 | 1.8 |
| Streptococcus salivarius | 2.8 | 5.8 | 0.0 | 6.2 | 2.5 | 3.8 | 1.8 | 0.1 | 0.1 |
| Actinomyces oris | 2.5 | 5.1 | 1.8 | 1.6 | 0.3 | 3.1 | 5.4 | 0.9 | 0.8 |
| Actinomyces meyeri | 2.2 | 4.5 | 2.2 | 2.0 | 0.1 | 4.0 | 6.4 | 0.3 | 0.7 |
| Streptococcus parasanguinis | 3.3 | 8.6 | 0.2 | 2.8 | 0.7 | 0.7 | 0.5 | 0.7 | 0.5 |
| TIMP1 | 0.3 | 12.2 | 0.0 | 2.2 | 0.0 | 5.7 | 1.8 | 1.0 | 0.3 |
| Streptococcus sanguinis | 1.0 | 11.2 | 0.3 | 0.8 | 0.0 | 5.3 | 2.8 | 1.0 | 0.0 |
| FEV1 | 0.7 | 9.3 | 0.1 | 0.5 | 0.3 | 4.7 | 5.5 | 2.8 | 3.2 |
| FVC | 0.6 | 7.5 | 0.6 | 4.3 | 0.2 | 1.3 | 3.1 | 0.5 | 2.1 |
| Stenotrophomonas maltophilia | 1.5 | 3.3 | 0.0 | 10.7 | 1.8 | 1.4 | 0.0 | 8.2 | 0.1 |
| Age | 0.6 | 7.6 | 0.6 | 8.1 | 0.0 | 0.4 | 0.1 | 1.5 | 1.1 |
| Haemophilus influenzae | 0.9 | 0.2 | 8.2 | 0.3 | 1.5 | 0.0 | 0.0 | 0.0 | 0.0 |
| Streptococcus thermophilus | 2.2 | 2.1 | 6.5 | 0.1 | 1.1 | 8.1 | 0.2 | 2.3 | 1.4 |
| Rothia dentocariosa | 1.2 | 0.6 | 10.4 | 3.9 | 0.6 | 5.4 | 6.9 | 0.6 | 0.8 |
| MMP9 | 1.1 | 0.7 | 9.9 | 3.1 | 0.8 | 4.9 | 5.8 | 0.9 | 1.9 |
| IL-1β | 1.1 | 0.7 | 11.5 | 2.5 | 0.7 | 6.2 | 7.1 | 0.6 | 0.3 |
| Streptococcus anginosus | 0.0 | 0.4 | 8.1 | 0.1 | 0.5 | 7.1 | 8.5 | 0.1 | 0.7 |
| MPO | 1.1 | 0.6 | 3.8 | 0.1 | 0.0 | 3.0 | 5.4 | 12.3 | 0.2 |
| Pseudomonas aeruginosa | 1.2 | 0.6 | 9.6 | 0.2 | 0.4 | 2.8 | 0.2 | 7.0 | 0.1 |
| Fusobacterium nucleatum | 0.7 | 0.6 | 2.6 | 2.4 | 7.9 | 2.2 | 0.5 | 6.6 | 1.4 |
| Veillonella parvula | 0.1 | 0.8 | 3.2 | 0.0 | 19.9 | 0.1 | 0.0 | 0.4 | 7.9 |
| Achromobacter xylosoxidans | 1.2 | 0.7 | 0.1 | 3.4 | 7.0 | 4.2 | 0.1 | 0.4 | 1.0 |
| Atopobium parvulum | 1.7 | 2.6 | 1.8 | 4.8 | 7.3 | 1.7 | 0.6 | 3.7 | 1.9 |
| IL-6 | 0.4 | 0.0 | 0.5 | 1.8 | 5.4 | 0.5 | 1.7 | 2.6 | 20.5 |
| MEF25 | 0.6 | 1.9 | 0.0 | 0.1 | 2.9 | 0.8 | 0.6 | 10.2 | 2.6 |
| Staphylococcus aureus | 1.6 | 0.8 | 0.6 | 2.7 | 1.1 | 0.7 | 2.0 | 16.9 | 1.0 |
| IL-8 | 0.3 | 0.0 | 0.0 | 0.1 | 0.9 | 0.6 | 4.4 | 1.6 | 31.6 |
| Bifidobacterium longum | 0.8 | 0.0 | 1.7 | 0.0 | 0.5 | 0.3 | 0.2 | 0.1 | 6.4 |
| Prevotella melaninogenica | 0.2 | 0.3 | 0.3 | 0.5 | 0.8 | 1.1 | 3.1 | 0.7 | 0.1 |
| Variability (%)b | 18.7 | 13.0 | 8.6 | 8.5 | 6.8 | 6.1 | 5.9 | 5.4 | 4.3 |
| Cumulative (%)c | 18.7 | 31.8 | 40.4 | 48.9 | 55.7 | 61.8 | 67.8 | 73.2 | 77.5 |
a
The selected clinical parameters are age and the lung function parameters FEV1, FVC, and MEF25. The selected host defense molecules are IL-1β, IL-6, IL-8, neutrophilic elastase (NE), myeloperoxidase (MPO), metalloproteinase 9 (MMP9), and metallopeptidase inhibitor 1 (TIMP1).
b
Explained variance by the respective principal component.
c
Sequential sum of explained variance sorted in ascending order.