TABLE 6.
Principal-component analysis of inflammatory parameters, clinical data, and the top bacterial species in sputum of 10 PS CF patientsa
| Parameter | Contribution (%) of observation in dimension |
||||
|---|---|---|---|---|---|
| F1 | F2 | F3 | F4 | F5 | |
| Actinomyces meyeri | 0.3 | 0.2 | 1.7 | 18.1 | 0.2 |
| IL-8 | 2.6 | 2.2 | 0.3 | 8.2 | 0.2 |
| Tannerella forsythia | 2.5 | 1.4 | 0.4 | 9.2 | 1.7 |
| Staphylococcus aureus | 0.7 | 0.0 | 1.7 | 7.9 | 3.4 |
| Bifidobacterium longum | 1.7 | 0.0 | 0.0 | 5.1 | 0.0 |
| Streptococcus anginosus | 0.6 | 0.3 | 0.1 | 4.8 | 1.9 |
| Achromobacter xylosoxidans | 0.8 | 1.2 | 1.4 | 3.7 | 2.2 |
| MPO | 0.7 | 0.0 | 9.5 | 0.2 | 1.3 |
| Fusobacterium nucleatum | 2.5 | 1.1 | 3.1 | 4.2 | 0.1 |
| Streptococcus pneumoniae | 0.3 | 1.0 | 4.7 | 4.1 | 4.1 |
| MMP9 | 1.8 | 0.8 | 3.4 | 0.1 | 11.2 |
| TIMP1 | 0.2 | 2.9 | 4.0 | 0.6 | 12.2 |
| Streptococcus gordonii | 0.3 | 10.6 | 0.6 | 1.0 | 1.1 |
| Pseudomonas aeruginosa | 0.2 | 8.2 | 0.9 | 3.1 | 0.0 |
| Haemophilus influenzae | 0.6 | 8.0 | 0.1 | 3.0 | 0.6 |
| Prevotella denticola | 0.6 | 7.0 | 0.0 | 2.7 | 0.5 |
| Streptococcus sanguinis | 0.3 | 10.0 | 0.3 | 3.4 | 0.8 |
| NE | 1.3 | 7.1 | 3.3 | 1.0 | 0.3 |
| Streptococcus thermophilus | 5.8 | 0.3 | 4.4 | 0.1 | 0.4 |
| Streptococcus salivarius | 5.5 | 0.0 | 5.7 | 0.3 | 0.2 |
| Streptococcus parasanguinis | 5.5 | 0.0 | 5.7 | 0.3 | 0.2 |
| Actinomyces oris | 5.5 | 0.0 | 5.8 | 0.3 | 0.2 |
| Atopobium parvulum | 5.5 | 0.0 | 5.8 | 0.3 | 0.2 |
| FVC | 5.4 | 0.0 | 5.8 | 0.3 | 0.2 |
| MEF25 | 4.8 | 0.3 | 6.9 | 0.0 | 0.0 |
| Age | 4.7 | 0.5 | 3.4 | 0.6 | 0.2 |
| Streptococcus oralis | 4.7 | 0.5 | 2.9 | 0.0 | 0.0 |
| Rothia mucilaginosa | 4.5 | 0.7 | 3.1 | 0.1 | 0.1 |
| Prevotella melaninogenica | 4.4 | 1.2 | 1.7 | 0.0 | 0.9 |
| Veillonella parvula | 4.4 | 1.3 | 0.0 | 0.0 | 10.3 |
| Cutibacterium acnes | 4.1 | 2.9 | 0.3 | 0.7 | 4.3 |
| Streptococcus mitis | 4.0 | 4.3 | 1.1 | 0.6 | 0.8 |
| FEV1 | 5.0 | 0.9 | 1.6 | 4.6 | 0.1 |
| IL-6 | 3.0 | 0.1 | 0.8 | 4.7 | 5.6 |
| Rothia dentocariosa | 1.2 | 0.7 | 0.0 | 0.9 | 4.6 |
| Haemophilus parainfluenzae | 1.0 | 1.9 | 0.1 | 0.0 | 19.5 |
| IL-1β | 2.9 | 0.5 | 1.6 | 0.0 | 0.0 |
| Variability (%)b | 28.3 | 17.8 | 14.2 | 10.3 | 9.2 |
| Cumulative (%)c | 28.3 | 46.1 | 60.2 | 70.5 | 79.7 |
a
The selected clinical parameters are age and the lung function parameters FEV1, FVC, and MEF25. The selected host defense molecules are interleukins IL-1β, IL-6, and IL-8, neutrophilic elastase (NE), myeloperoxidase (MPO), metalloproteinase 9 (MMP9), and metallopeptidase inhibitor 1 (TIMP1).
b
Explained variance by the respective principal component.
c
Sequential sum of explained variance sorted in ascending order.