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. 2023 Mar 30;14:1145763. doi: 10.3389/fphys.2023.1145763

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Copyright © 2023 Omi, Koneru, Lyu, Haraguchi, Kamiya and Mishina.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A) Top, H&E staining of the mouse proximal tibia. Scale bar = 100 µm. Bottom, TRAP staining of the mouse proximal tibia. Scale bar = 50 µm. (B) Bone area/tissue area (BA/TA), trabecular thickness (Tb.Th), trabecular number (Tb.N), trabecular space (Tb.Sp), osteoblast number/bone surface (N.Ob/BS), osteoblast surface/bone surface (Ob.S/BS), osteoclast number/bone surface (N.Oc/BS), osteoclast surface/bone surface (OcS/BS) were analyzed (n = 7 male for each group). ***p < 0.001, **p < 0.01.