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. 2023 Mar 30;15:1110425. doi: 10.3389/fnagi.2023.1110425

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Copyright © 2023 Riba, del Valle, Romera, Alsina, Molina-Porcel, Pelegrí and Vilaplana.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Representative images of wasteosomes from hippocampal sections of Alzheimer’s disease (AD) patients stained with the anti-p62 (red) and the Tau5 (green) antibodies after 30 min of boiling in PBS (first and second rows) or citrate buffer (third and fourth rows). Staining with the anti-p62 antibody showed the distinctive staining of wasteosomes. Tau staining colocalized with p62 staining in some areas of the wasteosomes (white arrowheads). p62 also colocalized with tau inclusions (white arrows). Hoechst staining (blue) was used for nuclear staining. Single channel (red and green) views are shown below merged images. Scale bar: 20 μm.