Fig 6.

The effect of DSS and dietary GSM treatment on the expression of genes coding for Keap1/Nrf2 signalling pathway in colon (A) and mesenteric lymph nodes (B). Unchallenged and DSS-treated pigs were assigned for 30 days to a Control diet (Control and DSS groups) or 8% GSM diet (GSM and DSS + GSM groups). At the end of the experiment, colon and lymph nodes samples from all animals (n = 5) were collected and analysed for gene expression (qPCR). ^{a, b, c =} Histograms for each group with unlike superscript letters were significantly different (p < 0.050). The heatmap (right panels) represents of Keap1, NQO1 and HO1 mRNA levels in colon (A-right panel) and mesenteric lymph nodes (B–right panel) collected from piglets. The blue and red colours correspond to low and high gene expression, respectively.