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. Author manuscript; available in PMC: 2023 Aug 1.
Published in final edited form as: Nat Cell Biol. 2023 Jan 19;25(2):298–308. doi: 10.1038/s41556-022-01060-1

Extended Data Fig. 6 |. Activation of ETV6 target genes specifically impairs Ewing sarcoma cell growth.

Extended Data Fig. 6 |

(a) RT-PCR analysis of SOX11 and NTRK1 levels in the CRISPR activation experiments. (−), no virus infection control. NT, non-targeting sgRNA control. Data are mean ± SEM. (n = 3 biological replicates) (b) Western blot confirming the elevation of SOX11 level upon CRISPR activation. ACTB was used as a loading control. (c) Competition-based proliferation assay evaluating the effects of candidate gene activation, using CRISPR activation (CRISPRa), to cell fitness in non-Ewing cancer cell lines (RD, U2OS and SUIT2). The percentage of GFP positive cells was monitored during culturing. Data are mean ± SEM. (n = 3 biological replicates) (d) Gene expression changes of SOX11 and NTRK1 in different cell lines assessed by RNA-seq. The significant expression changes (p