Primary human bronchial epithelial cultures that were established from donor lungs and grown at the air liquid interface were exposed to air, and aerosols generated by a third generation ENDS device containing either PGVG, PG/VG + nicotine, PG/VG + CBD oil, or PG/VG + CBD oil + VEA in 3 exposure sessions with 24 h intervals. Poste-exposure HBE cultures were analyzed by light microscopy, cell viability assessment and secretion proteomics.