Figure 1. Ahrr^−/− mice have reduced numbers of IEL.

(A) Numbers of CD45⁺ IEL in the small intestine of WT and Ahrr^−/− mice. (B-E) Numbers of T cells in small intestinal epithelium (B), small intestinal lamina propria (C), mesenteric lymph nodes (D) and spleen (E) of WT and Ahrr^−/− mice. (F) Representative flow cytometry plots depicting frequency of TCR-β⁺ IEL in WT and Ahrr^−/− mice. (G-J) IEL populations in small intestinal epithelium of WT and Ahrr^−/− mice, including TCR-γδ⁺ CD8αα⁺ (G), TCR-β⁺ CD8αα⁺ (H), TCR-β⁺ CD8αβ⁺ (I), and TCR-β⁺ CD4⁺ (J). (K) Immunofluorescence staining of CD8α in the small intestine of WT and Ahrr^−/− mice. (l) Quantification of CD8α+ cells per villus of WT and Ahrr^−/− mice. (M) Frequency of CD160⁺ TCR-β⁺ CD8αα⁺ IELs in WT and Ahrr^−/− mice. (N, O) Representative flow cytometry plots (N) and numbers of DP IEL (O) in WT and Ahrr^−/− mice (gated on CD45⁺, CD3⁺, TCR γδ⁻, TCRαβ⁺, CD8β⁻ CD4⁺ IEL). (P and Q) Representative flow cytometry plots plots (P) and frequency of ThPOK^low CD8αα⁺ cells (Q) in WT and Ahrr^−/− mice. Each dot represents an individual mouse. Data are pool or representative of 2–3 individual experiments. Statistical significance was determined by Mann-Whitney test. *P<0.05, **P<0.01. Please also see Figures S1 and S2.