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. Author manuscript; available in PMC: 2024 Apr 11.
Published in final edited form as: Immunity. 2023 Feb 22;56(4):829–846.e8. doi: 10.1016/j.immuni.2023.01.033

Figure 3: Mechanisms of RORγ+ Treg cell accumulation in regenerating muscle.

Figure 3:

(A) Egress of diverse lymphocyte populations from the colon, and (B) their emigration to spleen after 24 hr of colonic PhC of Kaede mice. Top: representative dot-plots; bottom: summary data. (C) Muscle RORγ+ Treg cell numbers after CTX-induced injury in vehicle- or FTY720-treated mice. (D) Colon PhC coupled with CTX-induced injury as per Figure 2C. Representative dot-plots (left) and summary data (right) of emigration of colonic cells to hindlimb muscles after 48 hr. (E) RORγ+ Treg cell fraction (left) and number (right) in hindlimb muscles 2 days after CTX-induced injury in in control- (Ctl), αCCL2-, or CP-105696-treated mice. (F, G) Mice were treated with isotype (IgG) or αMHC-II antibody. F) Muscle RORγ+ Treg cell fraction (left) and number (right), and G) Ki-67 expression 3 days after CTX-induced injury. Representative dot-plots are from 2–3 independent experiments. Unpaired t-test (A, B, F, G), two-way ANOVA (C), or one-way ANOVA (E). See also Figure S3.