Fig. 2. Evaluation of the high-confidence TDMD triggers.

a Genome browser view of trigger transcript models (blue boxes, exons; blue line, introns) with alternative isoforms and conservation plots, which are based on a 124 insects Basewise Conservation by PhyloP (phyloP124way). The transcripts are shown in the 5′ to 3′ direction. Red arrows point to the TDMD triggers. Diagramed below AGO1 transcript is the trigger site against miR-999 (in red), flanked by 15 nt on each side. The sequence logo based on six homologous sequences is shown from the indicated species. Asterisks indicate bases conserved in all of the representative examples. Tissue-specific miRNA abundance (b) and trigger transcripts abundance (c) in Drosophila from FlyAtlas 2. The gene abundance scale (log₂ FPKM for genes, log₂ RPM for miRNA) is shown as heatmap on the right. d Negative correlation between the miRNA abundance and trigger transcript abundance in Drosophila whole body. The linear regression is shown with solid line. r, pearson correlation coefficient. P = 0.0153 < 0.05 by two-tailed test. Source data are provided as a Source Data file.