Fig. 7. AGO1 trigger-KO flies are more vulnerable to stress.

The influence of TDMD trigger on miR-999 (a) and repression of miR-999 targets (b) in AGO1 trigger KO flies. Differences between each set of predicted targets and non-targets were assessed for statistical significance using the Mann–Whitney test (two-sided) and the associated P value. P value = 8.9 × 10⁻⁵ (<0.0001) for all predicted targets compared with non-targets, and P value = 7.1 × 10⁻⁵ (<0.0001) for conserved predicted targets compared with non-targets. c DAVID identified GO term biological pathways enriched in downregulated genes in AGO1 trigger KO flies compared with control-KO flies. Unadjusted P values were determined for the GO term analysis. d The expression of AGO1 after different treatments were generated by modENCODE of FlyBase. Five-day adult male and female are included to indicate baseline AGO1 expression. e Survival rate for AGO1 trigger KO flies after hydrogen peroxide exposure compared with control-KO flies. Data are presented as mean ± SD. (∗∗) P = 0.0094 < 0.01, t test. Four independent oxidative stress experiments were conducted. In each experiment, each fly line has three vials starting with 20 male flies. Source data are provided as a Source Data file.