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. 2023 Apr 13;13:6096. doi: 10.1038/s41598-023-33263-3

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Identification of individual G-tracts stabilized by helquat binding. (A) A Taq polymerase stop assay in the presence of 2.5 µM of the compounds being tested, with stop regions visible as bands of higher intensity. The sequence and numbered G-tracts (highlighted in red) are indicated on the left. The brackets labelled with letters A, B and C mark the regions selected for quantification. This figure was made by the excision of selected lines from Supplementary Fig. 2C, which shows the entire original gel. (B) Quantification of selected regions, normalized against the negative control (ctrl). The values represent a fold change calculated as ratio between pixel intensity of individual region A (G-patch 6′), B (G-patch 6) or C (G-patch 5) and pixel intensity of negative control (ctrl) of the same regions. The automatic background subtraction was performed by Rolling Ball method in 1D gel analysis using ImageQuant TL 8.1. (C) Quantification of all regions (A, B and C) together at different concentrations (see Supplementary Fig. S2 for gels) normalized against the control.The values represent a fold change calculated as ratio between pixel intensity of all regions (A, B, C) at different concentrations of compounds and pixel intensity of negative control (ctrl) of all regions (A, B, C). The same background subtraction method was used as in (B). n.a.—not analysed, PDS (pyridostatin)—positive control.