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. 2022 Nov 8;68(4):1260–1268. doi: 10.1007/s10620-022-07734-y

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License, which permits any non-commercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by-nc/4.0/.

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Fig. 1 — Immunohistochemistry and quantification of ICC, glial cells, and nNOS in LES tissue samples obtained from patients with achalasia and controls. The c‐kit staining was used to examine ICC, S-100β staining was used to assess glial cells and nNOS to nitrergic nerves. LES lower esophageal sphincter, ICC interstitial cells of Cajal, nNOS neuronal nitric oxide synthase. *P < 0.05. ns not significant