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. 2023 Feb 28;13(5):1470–1483. doi: 10.1007/s13346-023-01310-1

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© The Author(s) 2023

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — Penetration of Cy3-labeled nanoparticles in 3D spheroids using two-photon microscopy. A Schematics showing incubation of Cy3-PMC and Cy3-PNP with 3D 4T1 homospheroids and 4T1:3T3 heterospheroids. 4T1 cells were stained with CellTracker^TMGreen and 3T3 were stained with CellTracker.^TMBlue. B, C Two-photon microscopic images show the penetration of Cy3-PMC and Cy3-PNP, respectively and plots with the intensity profile of the red signal across the spheroids (shown with the dotted lines)