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. 2023 Feb 28;13(5):1470–1483. doi: 10.1007/s13346-023-01310-1

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© The Author(s) 2023

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — Efficacy study of PTX and PTX-loaded nanoparticles in 3D spheroids. Brightfield images showing the effect of PTX, PTX-loaded polymeric micelles (PTX-PMC), and PTX-loaded polymeric nanoparticles (PTX-PNP) on homospheroids (A) and heterospheroids (B). C–E % cell viability of 4T1 homospheroid and heterospheroid of 4T1:3T3 (1:5) after 48 h incubation with PTX formulations (C: free PTX, D: PTX-PMC, E: PTX-PNP). Cell debris was more prominent in PTX-PMC formulation (pointed by white arrow). Scale bar: 400 μm