Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Apr 13;14:2094. doi: 10.1038/s41467-023-37832-y

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 5 — a Confocal fluorescence microscopy images of PKH26-EV-encapsulated microspheres in the area of mouse myocardial infarction at 1, 7, 14, and 21 days, Scale bar = 50 μm. PKH26-EV (red fluorescence), DAPI (blue fluorescence). b CD68 immunofluorescence staining of macrophages in cardiac tissue on Day 7 after myocardial infarction, Scale bar = 50 μm. CD68 (green fluorescence) and DAPI (blue fluorescence). Quantitative analysis of the number of CD68-positive cells. c M-mode ultrasound images on Day 21 after myocardial infarction. d Quantification of the ejection fraction (EF) and fractional shortening (FS) of the animals on Day 21 after myocardial infarction. e Masson staining of hearts on Day 21 after myocardial infarction. f Statistical analysis of left ventricular wall thickness, scar thickness and infarct size. The number of hearts = 5. g Wheat germ agglutinin immunofluorescence staining of myocardial cells in the marginal zone of myocardial infarction on Day 21 after myocardial infarction, scale bar = 50 μm. Cross-sectional area measurements of cardiomyocytes in the marginal zone of myocardial infarction. h TUNEL staining in the infarct margin area on Day 21 after myocardial infarction, Scale bar = 100 μm. Total nuclei (DAPI staining, blue) and TUNEL-positive nuclei (tan). Quantitative analysis of TUNEL-positive cardiomyocytes. i Immunofluorescence staining pictures of small arteries in the peripheral area of myocardial infarction on Day 21 after myocardial infarction, scale bar = 50 μm. Alpha-smooth muscle actin (α-SMA, green fluorescence) and DAPI (blue fluorescence). Immunofluorescence staining of capillaries in the peripheral area of myocardial infarction on Day 21 after infarction. CD31 (red fluorescence) and DAPI (blue fluorescence). j Quantitative analysis of immunofluorescence staining of arterioles and capillaries. *p < 0.05 and **p < 0.01 vs. the PBS group; #p < 0.05, ##p < 0.01 vs. microsphere+CS-EPC-EVs. Data are presented as the mean ± standard. Two-tailed Student’s t-test was used to compare the differences between two groups. One-way ANOVA and post hoc Bonferroni tests were used to compare differences among more than two groups. Source data are provided as a Source Data file. Each experiment was repeated 3 times or more independently with similar results.