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. 2023 Apr 13;14:2094. doi: 10.1038/s41467-023-37832-y

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a Schematic of coincubation. This Figure was created with BioRender.com. b Transwell experiments and quantitative analysis of EPCs with different conditioned media under normoxia. *p < 0.05, **p < 0.01 vs. OGD; #p < 0.05, ##p < 0.01 vs. CS-EV+CM (n = 3, biological replicates per group), scale bar = 50 μm. c Cluster of Differentiation 34/Vascular Endothelial Growth Factor Receptor 2 (CD34+/VEGFR2+) immunofluorescence staining of EPCs in the peripheral area of myocardial infarction on Day 7 after infarction. CD34+ (red), VEGFR2+ (green), DAPI (blue), CD34+/VEGFR2+ (orange), scale bar = 50 μm, specimens (n = 5, biological replicates per group). d Fluorescence images of 3,3’-Dioctadecyloxacarbocyanine Perchlorate-EPCs (DiO-EPCs) and CD31-positive capillaries in the peripheral area of myocardial infarction on Day 7 after infarction. (1) and (2) are images of the corresponding area zoomed in on the yellow dot box. PKH26-EV (red fluorescence, white arrow), DiO-EPC (green fluorescence, purple arrow), CD31 (white fluorescence, yellow arrow), and DAPI (blue fluorescence) (n = 5, biological replicates per group). e Quantitative analysis of DiO-EPCs and the number of capillaries in the peripheral area of myocardial infarction (200 x magnification). *p < 0.05, **p < 0.01 vs. the PBS group; #p < 0.05, ##p < 0.01 vs. microsphere+CS-EPC-EVs (n = 5, biological replicates per group). f Schematic diagram of the DiO-EPCs injection and microsphere+CS-EPC-EV treatment of infarcted nude mice. This Figure was created with BioRender.com. Data are presented as the mean ± standard. Two-tailed Student’s t-test was used to compare the differences between two groups. One-way ANOVA and post hoc Bonferroni tests were used to compare differences among more than two groups. Source data are provided as a Source Data file. Each experiment was repeated 3 times or more independently with similar results.