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. 2023 Apr 13;14:2094. doi: 10.1038/s41467-023-37832-y

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 8 — a qRT‒PCR analysis of miR-126a-3p expression in CS-EPCs after miR-126a-3p inhibitor transfection. b qRT‒PCR analysis of miR-126a-3p expression in EVs derived from miR-126a-3p inhibitor-transfected EPCs and verification of whether EVs mediate angiogenesis in HUVECs by transferring miR-126a-3p. EPCs were transfected with miR-126a-3p inhibitor (miR-126a-3p inhibitor NC was used as a negative control group) to silence the effect of miR-126a-3p and then cultured with silicate ions (CS+126IEPC-EV). c, d Qualitative and quantitative analysis of scratch migration experiments (scale bar = 150 μm), transwell migration experiments (scale bar = 100 μm) (c) and tube formation experiments (scale bar = 150 μm) (d) of HUVECs treated with different EVs under glucose and oxygen deprivation. e Qualitative and quantitative analysis of Transwell migration experiments of EPCs treated with different conditioned media under normoxic conditions, scale bar = 100 μm. f qRT‒PCR analysis of the expression of SDF-1 and eNOS in HUVECs treated with different EVs under glucose and oxygen deprivation. g The expression and quantitative analysis of RGS16 and CXCR4 proteins in HUVECs treated with different EVs under glucose and oxygen deprivation. h The expression and quantitative analysis of Regulator of G protein Signaling 16 (RGS16), C-X-C Chemokine Receptor Type 4 (CXCR4), SDF-1, phosphorylated protein kinase B (p-AKT) and total protein kinase B (t-AKT) proteins in HUVECs treated with different EVs under glucose and oxygen deprivation. *p < 0.05 and **p < 0.01 vs. OGD, #p < 0.05 vs. the other two groups. Data are presented as the mean ± standard. Two-tailed Student’s t-test was used to compare the differences between two groups. One-way ANOVA and post hoc Bonferroni tests were used to compare differences among more than two groups. Source data are provided as a Source Data file. Each experiment was repeated 3 times or more independently with similar results.