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. 2023 Apr 13;14:2072. doi: 10.1038/s41467-023-37519-4

Fig. 2. AlphaFold2-Multimer based molecular model of the Xenopus laevis augmin complex.

Fig. 2

a AlphaFold2-Multimer prediction of X. laevis augmin subcomplexes—T-II (comprised of Haus2, Haus61–430, Haus7, and Haus8) and T-III (comprised of Haus1, Haus3, Haus4, and Haus5)—can be manually docked, then rigid-body fit into the 6.9 Å cryo-EM map to give good overall agreement. b A widening of the T-III stalk can be well modeled by a helical hairpin insertion in Haus5. c A bulge halfway down the T-III stalk can be modeled by α-helical bundles predicted at the N-termini of Haus1 and Haus4. d The second leg of T-III, comprising the extreme N-terminus of Haus3 and Haus5, has no corresponding density in the cryo-EM map, perhaps due to flexibility. e The globular domains at the N-termini of Haus6 and Haus7 imperfectly fit the cryo-EM map, perhaps a result of multiple conformational states.