Figure 2.

Induction of severe colitis in TCRδ^-/- mice induces neutrophilic lung inflammation. (A) Representative FACS plots showing frequencies of CD45⁺ cells vs side scatter (SSC-A) in digested whole lung tissue of C57BL/6 or TCRδ^-/- mice treated with either H₂O or DSS. (B) Total number of CD45⁺ cells in whole lung tissue as determined by cell counts and flow cytometry from C57BL/6 (circle) or TCRδ^-/- mice (triangle) treated with either H₂O (closed) or DSS (open). n = 7 – 8 mice per group. (C) Representative FACS plots depicting frequencies of neutrophils (CD45⁺CD11c^-CD11b⁺SiglecF^-Gr-1⁺) and eosinophils (CD45⁺CD11c^-CD11b⁺SiglecF⁺Gr-1^-) gated on CD45⁺CD11c^-CD11b⁺ cells in whole lung. (D) Total number of neutrophils, eosinophils, and alveolar macrophages (CD45⁺CD11c⁺SiglecF⁺) in whole lung tissue derived by flow cytometry and cell counting. n = 7 – 8 mice per group. (E) Geometric mean fluorescence intensity (gMFI) of expression of Gr-1, CD11b, and CD45, and histogram of Gr-1 on lung neutrophils relative to C57BL/6 H₂O. n = 7 – 8 mice per group. (F) Representative FACS plots depicting frequencies of CD4⁺ and CD8⁺ T cells gated on CD45⁺CD3⁺γδTCR^- lymphocytes in whole lung. (G) Total number of CD4⁺ and CD8⁺ T cells in whole lung tissue derived by flow cytometry and cell counting. n = 7 – 8 mice per group. Data is presented as median ± IQR. ns, not significant; *p < 0.05; **p < 0.01; ***p < 0.001 by Mann-Whitney U test comparing water and DSS-treated mice of the same genotype.