Skip to main content
. 2023 Mar 31;14:1125260. doi: 10.3389/fimmu.2023.1125260

Figure 4.

Figure 4

DSS-induced colitis in TCRδ-/- mice promotes lung disease. (A) Total BAL cell counts from C57BL/6 (circle) or TCRδ-/- mice (triangle) treated with either H2O (closed) or DSS (open). n = 13 – 17 mice per group. (B) Representative cytospins of BAL cells of the indicated mice stained with Hemacolor® captured at 40x magnification. Scale bar = 50 μm. (C) Proportion of alveolar macrophages (CD45+CD11c+SiglecF+), neutrophils (CD45+CD11b+Ly6G+), and eosinophils (CD45+CD11b+SiglecF+) in BAL as determined by flow cytometry. n = 5 mice per group. (D) H&E-stained sections of inflation-fixed lungs of the indicated mice captured at 20x magnification. Scale bars = 100 μm. (E) Alveolar airspace size defined as mean linear intercept (MLI) in μm. n = 10 – 15 mice per group. Data is presented as median ± IQR. ns, not significant; *p < 0.05; ****p < 0.0001 by Mann-Whitney U test comparing water and DSS-treated mice of the same genotype (A, D).