Fig. 1.
Characterization of collagen IV-targeted lipopolyplexes encapsulating the shRNA plasmid. Average hydrodynamic diameter and polydispersity indices (A-a) and Zeta potential (A-b) of collagen IV-targeted lipopolyplexes (Cp-LPP/shCtr) and non-targeted lipopolyplexes (S-LPP/shCtr); results are reported as mean ± S.D. for three individual measurements. Cytotoxicity assay evaluated as adenylate kinase (AK) release in the culture medium at 48 h after transfection of oVIC seeded on 2D support (B-a) and in 3D aortic root-derived hydrogel (B-b) performed on the 5th and 12th day; data are presented as mean ± S.D. of four experiments done in duplicates.