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. 2023 Mar 28;20:100620. doi: 10.1016/j.mtbio.2023.100620

Fig. 6.

Fig. 6

Cp-LPP/shRunx2 lipopolyplexes reduce the ALP activity and block the formation of calcium deposits. (A) Quantitative assay of ALP activity performed at 7 and 14 days after a single transfection of HGOM-activated VIC on day 5 or two transfections (on days 5 and 12) with different nanoparticle formulations. (B) Quantitative assay of calcium concentration at 7 and 14 days after a single transfection of HGOM-activated VIC on day 5 or two transfections (on days 5 and 12) with different nanoparticle formulations. For both assays, data are means ​± ​S.D. of three experiments in triplicate and presented as fold change relative to HGOM condition (considered 1). ∗p ​< ​0.05, ∗∗p ​< ​0.01, ∗∗∗p ​< ​0.001, ∗∗∗∗p ​< ​0.0001 when compared with HGOM at 7 days; #p ​< ​0.05, ##p ​< ​0.01, ###p ​< ​0.001, ####p ​< ​0.0001 when compared with HGOM at 14 days. (C) Alizarin Red-stained calcium deposits in 3D-cultured VIC after 14 days of HGOM exposure and two transfections performed on days 5 and 12 with different nanoparticles. The graph displays the quantitative measurement of the dye released from the hydrogel. Data are means ​± ​S.D. of two experiments made in triplicates and were represented as fold change relative to HGOM condition (considered 1). ∗∗p ​< ​0.01, ∗∗∗p ​< ​0.001 when compared with HGOM; #p ​< ​0.05, ##p ​< ​0.01 when compared with NM.