Figure 2.

Anti-TIGIT RPs abrogated TIGIT expression in primary human NK cells

(A) Representative mean fluorescence intensity (MFI) of TIGIT surface expression in human pNK cells transduced with RPs targeting the TIGIT gene (n = 3). Data represent mean ± SD. (B) Representative histograms depicting the expression of various surface receptors on pNK cells transduced by RPs. (C) Sequences of the whole TIGIT PCR fragments from pNK cells treated with RPs. The arrow indicates a mutation starting site analyzed by Sanger sequencing. (D) ICE analysis of sequenced TIGIT PCR fragments from three donors that received CRISPR-Cas9 and sgRNA-loaded RPs. The upper chromatograms show sequencing results (Cas9-RNP-RP samples, top; non-treated controls, bottom). The sequence signal plots show the discordant sequences between control (orange) and RP-received samples (green). The right bar graphs indicate frequencies of indel mutations. Eff, editing efficiency; ns, non-significant; ∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001; ∗∗∗∗p < 0.0001.