Figure 5.

Site-specific CAR integration into an NK cell genome by Cas9-sgRNA RPs

(A) Illustrative concept of a site-specific CAR integration into a genome by Cas9-sgRNAs. The bottom DNA indicates the genomic modification after the engineering using Cas9-RNP-loaded RPs. Arrows indicate the locations and directions of primers for PCR. (B) PCR amplicons from PCR using primer set 1, set 2, and set 3. Note that the amplicons from PCR with primer set 3 indicate possible site-specific CAR integration into the TIGIT locus. (C) Representative Sanger sequencing results of three independent amplicons from PCR with primer set 3. (D) Summary of various genomic events in seven independent amplicons from PCR with primer set 3.