TABLE 1.

The primer sequences.

Target gene		Primer sequence (5′to 3′)		Size (bp)
Name	Id	Forward	Reverse
ACSL4	2,182	ATA​CCT​GGA​CTG​GGA​CCG​AA	TGC​TGG​ACT​GGT​CAG​AGA​GT	145
TfR1	7,037	TGG​AGA​CTT​TGG​ATC​GGT​TGG​TG	CAG​TGG​CTG​GCA​GAA​ACC​TTG	138
NCOA4	8031	GGG​CAA​CCT​CAG​CCA​GTT​AT	CAA​ACT​GCA​GGG​AGG​CCA​TA	139
FTH1	2,495	CCA​GAA​CTA​CCA​CCA​GGA​CTC	CAA​AGC​CAC​ATC​ATC​GCG​G	118
GPX4	2,879	CAG​TGA​GGC​AAG​ACC​GAA​GT	CCG​AAC​TGG​TTA​CAC​GGG​AA	104
xCT	23657	CAT​CTC​TCC​TAA​GGG​CGT​GC	TAG​TGA​CAG​GAC​CCC​ACA​CA	85
H O -1	3162	CCG​CAT​GAA​CTC​CCT​GGA​GAT​G	CTG​GAT​GTT​GAG​CAG​GAA​CGC​AG	85
β-actin	60	CCTGGCACCCAGCACAAT	GGGCCGGACTCGTCATAC	114

ACSL4: acyl-CoA, synthetase long-chain family member 4; NCOA4: nuclear receptor coactivator 4; FTH1: ferritin heavy chain 1; GPX4: glutathione peroxidase 4; H O -1: heme oxygenase 1; xCT: cystine-glutamate antiporter; TfR1: transferrin receptor 1.