Figure 1.

Cisplatin increases the levels and expression of CXCL1 in the cochlea. Male Wistar rats were treated with vehicle or cisplatin (11mg/kg) and sacrificed (A) 1 day later and cochleae extracted and processed for CXCL1 using ELISA. (B) Quantification of CXCL1 mRNA in the cochlea show significant elevations by 6 h and which remained elevated at least up to 72 h (P<0.0001, F(5,18)=23.20 and Degree of Freedom (D.F.)=23). (C) Mid-modiolar sections of cochleae showing CXCL1 immunolabeling over time. Prominent labeling was observed in the organ of Corti (OC) and spiral ganglion (SG). Increased labeling was also observed in the spiral limbus (SLi),spiral ganglion,organ of Corti and auditory nerve. CXCL1 staining was further increased in these regions by 48 h. By 72 h, labeling of CXCL1 in the spiral limbus, auditory nerve, spiral ganglion neurons and organ of Corti was further increased. Some intense labeling of cells is seen in the region of the spiral ligament/spiral prominence. Open arrows indicate OHCs and IHCs. Solid arrows show representative locations where increases in CXCL1 were observed. Asterisk (*) indicates statistical significance from vehicle-treated rats which was determined by analysis of variance (one-way ANOVA). The data is plotted as mean ± SEM (n ≥ 6). Scale bars – black = 50 µm, red = 20 µm.