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. 2023 Mar 31;14:1125948. doi: 10.3389/fimmu.2023.1125948

Figure 11.

Figure 11

SB225002 and EGCG modulate cisplatin-induced inflammatory gene in UB/OC-1. (A) UB/OC-1 cells were pretreated with vehicle or SB225002 (300 nm) for 30 min, followed by cisplatin treatment (2.5 µM) for 24 hand RNA was isolated. The expression of the inflammatory genes CXCR2, CXCR1, CXCL1, TNFα and COX2 were determined by q-PCR using GAPDH as an internal control (housekeeping gene) for normalization. Cisplatin increased the expression of these genes while SB225002, an inhibitor of CXCR2, significantly reduced the induction of these genes (P<0.0009, F(3,16)=9.195 and DF=19). Data are presented as the mean fold change ± SEM (n=4). Asterisk, (*) indicates significant difference (p<0.05) from vehicle group while (**) indicate statistically significant difference from cisplatin-treated group. Statistical analyses among groups were tested using one-way analysis of variance (ANOVA). (B) UB/OC-1 cells were treated with vehicle or EGCG (100 µM) for 30 min followed by cisplatin (2.5 µM) for 24 h. and RNA was isolated using TRI reagent. The expression level of the inflammatory genes CXCR2, CXCR1, CXCL1, TNFα, and COX2 were determined by qRT-PCR, GAPDH was used as internal control (housekeeping gene) for gene expression normalization. Cisplatin was increased expression of inflammatory genes while EGCG showed significant protection against cisplatin effects (P<0.0007, F(3,16)=11.1 and DF=19). Data were presented fold change mean ± SEM (n≥3). (C) UB/OC-1 cells were treated with vehicle or celecoxib (50 µM) for 30 min followed by cisplatin (2.5 µM) for 24 h, RNA was isolated using TRI reagent. The expression level of the inflammatory genes CXCR2, CXCR1, CXCL1, TNFα and COX2 were determined by qRT-PCR, GAPDH was used as internal control (housekeeping gene) for gene expression normalization. Cisplatin was increased expression of inflammatory genes while Celecoxib showed significant protection against cisplatin effects (P<0.0001, F(3,16)=80.1 and DF=19). Data were presented fold change mean ± SEM (n≥3). Asterisks, (*) indicates significant difference (p<0.05) from vehicle group, while (**) indicate significant difference (p<0.05) form cisplatin group. Statistical significance of differences among groups were tested using one -way analysis of variance (ANOVA).