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. 2023 Mar 31;14:1125948. doi: 10.3389/fimmu.2023.1125948

Figure 3.

Figure 3

Cisplatin increases the expression of chemokine/chemokine receptors and other inflammatory markers in the cochlea. Male Wistar rats were administered intraperitoneal cisplatin (11mg/kg) and the induction of inflammatory genes were assessed using time course studies for 6, 12, 24, 48, 72 h. The expression of cochlear CXCR2, CXCR1, CXCL1, TNFα and COX2 genes were determined by q-PCR using GAPDH as an internal control (housekeeping gene) for normalization. Cisplatin significantly increased CXCR2, CXCR1, CXCL1, TNFα and COX2 expression after 6 h while STAT1 was increased only after 12 h of treatment (P<0.0003, F(5,25)=7.273 D.F. = 35). The level of STAT3 expression showed no change after 6 h of cisplatin treatment, but was significantly decreased by 12 h. Asterisk (*) indicates statistical significance from vehicle-treated rats which was determined by analysis of variance (one-way ANOVA). The data is plotted as mean ± SEM (n ≥ 6).