Safety assessment of sonobiopsy. (A) Representative
hematoxylin-eosin staining for experiment 1 with 2-month-old mice.
(B) There was a significant increase in microhemorrhage
surface area (red arrow) in the treated hemisphere (mean, 0.051
mm2 ± 0.039) compared with the untreated
hemisphere (mean, 0.016 mm2 ± 0.018;
P = .036). (C) Representative
hematoxylin-eosin staining for experiment 2 with 6-month-old mice.
(D) There was no significant difference in the
microhemorrhage density in the treated hemisphere (mean, 0.014
mm2 ± 0.006) compared with the untreated
hemisphere (mean, 0.013 mm2 ± 0.007;
P = .26; n = 13). Minimal
visualized microhemorrhage in the untreated contralateral cerebral
hemisphere likely represents artifactual red blood cell extravasation
during perfusion, tissue handling, and fixation.