Figure 7. Voluntary running does not mitigate the loss of Oflr160-axonal projections and expressing OSNs elicited by consumption of a fatty diet.

A, Representative whole-mount images of olfactory bulbs following beta-galactosidase histological staining of Olfr160-expressing olfactory sensory neurons (OSNs) from male M72tauLacZ mice following diet/exercise treatments; images from left to right: control diet ad libitum (brown food symbol), control diet plus running wheel (brown food and wheel symbol), moderately high-fat diet ad libitum (pink food symbol), moderately high-fat diet plus running wheel (pink food and wheel symbol). Projections were converted to pixel density and OSNs manually counted as in Figs. 2/3. o = olfactory bulb, g = glomerulus, and a = Olfr160 axonal projections. Bar graph of B, pixel density of axonal projections and C, number of Olfr160-expressing OSNs vs. diet/exercise treatment; CF Sed = control diet sedentary, CF VEx = control diet voluntary exercise, MHF = moderately high-fat diet sedentary, MHF Vex = moderately high-fat diet voluntary exercise. D, Line graph of body weight vs. age; 2-way RM ANOVA; symbols indicate significantly different for treatment factor as determined by Tukey’s post-hoc (p < 0.05) as follows: # = MHF Sed > both CF groups; ♦ = MHF VEx > both CF groups; * = MHF Sed > MHF VEx. E, Bar graph of body weight vs. diet/exercise treatment at 25 weeks of age. F, Line graph of the plasma glucose concentration and G, bar graph of the iAUC during the IPGTT. H, Bar graph of the total excised adipose tissue vs. diet/exercise treatment. Notations as in Fig. 2. (B, C, E, G, H) 1-way ANOVA, Tukey’s post-hoc, p < 0.05. Sample sizes in number of mice from left to right for whole mount: 14, 9, 12, 9 | for neuronal counts: 11, 8, 10, 8 | for body weight and IPGTT: 14, 9, 12, 9 | and for adipose tissue = 11, 9, 12, 9.