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. 2023 Feb 13;83(8):1214–1233. doi: 10.1158/0008-5472.CAN-22-1769

Figure 4.

Figure 4. Expression correlation of myeloma cell–associated genes encoding surface proteins. A, UMAP showing plasma cells from 53 patients reveal tumor-specific clusters, colored by patient. B, Dot plots showing correlation of gene expression averaged by sample in all 53 samples, colored by Pearson correlation coefficient (r value). Only predicted coexpressed gene pairs (r>0.30) and mutually exclusively expressed gene pairs (r<0.05) are shown. Coexpressed gene groups are highlighted in triangles with visually confirmed ones listed on the right. Bar charts showing the number of predicted gene pairs (r<0.05 or r>0.30) in 2 data sets or 3 data sets. C, Gene pairs showing mutually exclusive expressions (top row) or coexpressions (bottom row). The first box of each row shows an example of expression of gene A (first UMAP), expression of gene B (second UMAP), and dual expression of gene A and gene B (third UMAP). D, Bar chart showing the percentage of plasma cells from 12 patient samples (11 MM and 1 SMM) with coexpression of surface proteins detected by flow cytometry. E, Pearson correlation plots of gene pair (same genes shown in D) expression in scRNA-seq.

Expression correlation of myeloma cell–associated genes encoding surface proteins. A, UMAP showing plasma cells from 53 patients reveals tumor-specific clusters, colored by patient. B, Dot plots showing correlation of gene expression averaged by sample in all 53 samples, colored by Pearson correlation coefficient (r value). Only predicted coexpressed gene pairs (r > 0.30) and mutually exclusively expressed gene pairs (r < 0.05) are shown. Coexpressed gene groups are highlighted in triangles, with visually confirmed ones listed on the right. Bar charts showing the number of predicted gene pairs (r < 0.05 or r > 0.30) in two data sets or three data sets. C, Gene pairs showing mutually exclusive expressions (top row) or coexpressions (bottom row). The first box of each row shows an example of expression of gene A (first UMAP), expression of gene B (second UMAP), and dual expression of gene A and gene B (third UMAP). D, Bar chart showing the percentage of plasma cells from 12 patient samples (11 MM and 1 SMM), with coexpression of surface proteins detected by flow cytometry. E, Pearson correlation plots of gene pair (same genes shown in D) expression in scRNA-seq.