Fig. 1.

Schematic representation of the experimental conditions within the acoustic startle reflex and PPI assays. a Mice were exposed to a pseudo-random presentation of five trial types (13 pulse-only trials, 13 of each three prepulse + pulse trials, and 13 no pulse trials for a total of 65 trials). In addition, the pulse-only trial was presented five times at the beginning and end of the testing session. b–e Mice were exposed to up to four different experimental conditions that included changes in background and stimulus intensity, prepulse and pulse duration, and interstimulus interval mean. Statistical significance of experiments involving three or more groups and two or more treatments was assessed by two-way or three-way ANOVA followed by Sidak’s multiple comparison test. Statistical significance of experiments involving time courses and two treatments was assessed by two-way repeated measures ANOVA. Statistical significance of experiments involving three or more groups was assessed by one-way ANOVA followed by Sidak’s multiple comparison test. Statistical significance of experiments involving two groups was assessed by Student’s t test. Correlation analysis was conducted using the Pearson’s r. Statistical analysis was performed with GraphPad Prism software version 9 (La Jolla, CA). The level of significance was set atp = 0.05. All values in the figure legends represent mean ± SEM.