Table 5.

pH and fermentation products in the ileum and colon of weaned piglets (day 14 post-weaning) according to dietary CP level and BM supplementation

	Treatment1				CP level2		BM suplementation2			P-value
	S0	S1	L0	L1	S	L	0	1	RSE	CP level	BM supplementation	Interaction
Ileum
pH	6.47	6.79	6.63	6.54	6.59	6.59	6.55	6.62	0.285	0.976	0.643	0.269
NH3, μm/g	1.43	1.69	1.21	1.80	1.56	1.51	1.32	1.75	0.720	0.912	0.235	0.693
Total lactate, µmol/g	35.7	25.8	37.1	30.0	30.8	33.5	36.4	27.9	1.761	0.755	0.342	0.876
Total SCFA, µmol/g	5.40	8.34	5.57	5.93	6.87	5.75	5.49	7.14	2.646	0.405	0.223	0.340
Total acetate, %	86.8	98.6	98.7	73.1	92.6	85.9	92.6	85.8	20.06	0.510	0.503	0.072
Total butyrate, %	0.93	1.42	1.31	1.94	1.18	1.63	1.12	1.68	2.023	0.658	0.581	0.948
Colon
pH	6.17	6.09	6.09	5.9	6.13	6.00	6.13	6.00	0.209	0.194	0.219	0.570
NH3, μm/g	13.6	12.3	7.10	12.9	12.9	9.98	10.3	12.6	9.63	0.484	0.722	0.411
Total lactate, µmol/g	5.56	4.04	4.34	3.33	4.80	3.84	4.95	3.69	4.542	0.673	0.581	0.911
Total SCFA, µmol/g	118	130	127	126	124	126	122	128	13.28	0.694	0.397	0.291
Total acetate, %	55.3	59.2	55.9	57.3	57.2	56.6	55.6	58.2	3.004	0.661	0.088	0.407
Total propionate, %	24.5	22.4	23.7	24.7	23.4	24.2	24.1	23.5	1.856	0.411	0.555	0.102
Total butyrate, %	14.6	14.3	14.9	14.3	14.5	14.6	14.8	14.3	2.095	0.878	0.634	0.892
Total valerate, %	3.41	2.26	3.41	2.42	2.84	2.92	3.41	2.34	0.625	0.811	0.002	0.804
Total BCFA, %	2.22	1.93	2.12	1.34	2.08	1.73	2.17	1.64	0.676	0.313	0.125	0.478

0, non-supplemented diet; 1, diet supplemented with BM at 1 kg/t; S0, S diet not supplemented; BCFA, branched chain fatty acids; BM: sodium butyrate protected by medium-chain fatty acid salts, DICOSAN (Norel S.A., Madrid, Spain); L, low dietary CP level (18.8%); L0, L diet not supplemented; L1, L diet supplemented with BM at 1 kg/t; NH₃, ammonia; RSE, residual standard error; S, standard dietary CP level (22.2%); S1, S diet supplemented with BM at 1kg/t; SCFA, short-chain fatty acids;.

¹Values are means for 8 pens.

²Values are means for 16 pens.