Figure. 1.

IGV alignment view of ALVE1 and ALVE_ros276 in White Leghorn and Tibetan, respectively(A). TSD: Target site duplication. (B) PCR diagnostic for ALVE_ros276. M: Marker. (−) 621 bp, (+) 100 bp. lane 1 and 4 was a heterozygous insertion, lane 2 and 3 was no insertion, and lane 5 was a homozygous insertion. (C) PCR diagnostic for ALVE TYR in Silkies, Cornish, and White Plymouth Rock. (+) 345 bp, all lanes were homozygous insertion. (D) The insertion of ALVE21 in White Leghorn. (+) 390 bp, (−) 510 bp, all lanes were heterozygous insertion. (E) The diagram for comparison of sequences from ALVE_ros276, ALVE_ros127, and ALVE_CAU005 with that from ALVE1. SNPs were marked by arrows. The intergenics represented non-coding regions.