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. Author manuscript; available in PMC: 2023 Apr 14.
Published in final edited form as: Immunohorizons. 2022 Dec 1;6(12):872–882. doi: 10.4049/immunohorizons.2100112

Figure 4. STAT3 is required for IL-6 induction of Pdcd1 in CD8 T cells.

Figure 4.

(A) Schematic of experimental outline. Primary CD8 T cells were isolated from spleens of Stat3fl/flGzmbCRE conditional knockout (KO) mice and WT counterparts and stimulated ex vivo with anti-CD3/CD28 beads in the presence and absence of IL-6 for 4 days. (B) Pdcd1 and Stat3 mRNA expression measured by qRT-PCR every 24 hours exposed to treatment conditions detailed in (A). Data are representative of three independent experiments and plotted ± SEM. Statistical significance was determined by two-way ANOVA: *p < 0.05, **p < 0.01, ***p < 0.001.