Figure 1.

Analysis of human immunoglobulin binding to pig PBMC from genetically modified swine. PBMC were collected, incubated with human serum, and analyzed for IgM (y axes) and IgG (x axes) binding by flow cytometry. Dotted lines represent an MFI of 2000. (A) A single representative patient is screened on wild type, GGTA1 KO, GGTA1/CMAH KO, and GGTA1/CMAH/b4GalNt2 KO swine PBMC. (B) Sera from 44 randomly selected patients with unknown sensitization were incubated with PBMC from all four glycan backgrounds.