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. 2023 Apr 14;18:16. doi: 10.1186/s13062-023-00371-z

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© The Author(s) 2023

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — USP5 promotes H1299 and A549 cells proliferation and migration. (A) The expression levels of USP5 via RNA interference were checked by Western blotting. (B) MTT assay was performed to examine the cell viability after knocking down USP5. (C-D) Colony-forming assay represents the proliferation ability of H1299 and A549 cells after downregulation of USP5. (E) Ctrl and USP5 overexpressed H1299 and A549 cells were subjected to Western blotting analysis of USP5. (F-H) Proliferation, colony formation and migration abilities were analyzed in H1299 and A549 cells after over-expression of USP5. * P < 0.05, and ** P < 0.01